Methylene blue phosphoramidite for DNA labelling.
نویسندگان
چکیده
We report the first synthesis of a methylene blue (MB) phosphoramidite derivative suitable for DNA solid-phase synthesis. The electrochemical and optical properties of the resulting MB modified oligonucleotides were confirmed. This new molecule is an important breakthrough in the design of new probes labelled with MB.
منابع مشابه
A novel optical DNA biosensor for detection of trace concentration of Methylene blue using Gold nano-particles and Guanine rich single strand DNA
The glass surface modification with 3-(mercaptopropyl) trimethoxysilane (MPTS), gold nano-particles (GN) and guanine rich single strand DNA (ss-DNA) was utilized as a novel and efficient platform for sensing trace concentration of methylene blue (MB) by an inexpensive spectrophotometric method. Methylene Blue (MB) can interact with the guanine base of single strand DNA and absorbed onto glass s...
متن کاملA novel optical DNA biosensor for detection of trace concentration of Methylene blue using Gold nano-particles and Guanine rich single strand DNA
The glass surface modification with 3-(mercaptopropyl) trimethoxysilane (MPTS), gold nano-particles (GN) and guanine rich single strand DNA (ss-DNA) was utilized as a novel and efficient platform for sensing trace concentration of methylene blue (MB) by an inexpensive spectrophotometric method. Methylene Blue (MB) can interact with the guanine base of single strand DNA and absorbed onto glass s...
متن کاملOne-step labelling of oligonucleotides with fluoresceine during automated synthesis.
Fluorescent or biotin labelling of an oligonucleotide at it's 5'-end usually involves two steps. First, a N-protected aminoalkyl phosphoramidite derivative (as for instance Aminolink 2 from Applied Biosystems) is added to the 5'-end of an oligonucleotide during automated DNA synthesis. After removal of all protecting groups the NHS ester of an appropriate fluorescent dye or biotin is coupled to...
متن کاملCT-guided methylene-blue labelling before thoracoscopic resection of pulmonary nodules.
OBJECTIVE Evaluation of the efficiency of our technique of methylene-blue labelling of pulmonary nodules to facilitate thoracoscopic recognition and excision. DESIGN Patients with a peripheral pulmonary nodule smaller than 2.5 cm and not in contact with the visceral pleura were included. Under tomodensitometric guidance, the nodules were labelled with methylene-blue within hours before thorac...
متن کاملMethylene Blue Prevents Post-Surgical Adhesion Formation in the Rabbit
Peritoneal adhesions are challenging in abdominal surgery. In spite of different kinds of treatments still no definitive and effective treatment is suggested. To determine the prevention effects of intraperitoneal (ip) methylene blue in post surgical adhesion formation 44 rabbits were randomly divided in two groups of equal number. Group I received an intra peritoneal injection of a 1% suspensi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 51 21 شماره
صفحات -
تاریخ انتشار 2015